IBD Patients Have More DNA Damage in Lymphocytes, Less Vitamin B6, Study Reports

IBD Patients Have More DNA Damage in Lymphocytes, Less Vitamin B6, Study Reports
Patients with inflammatory bowel disease (IBD) have greater DNA damage in lymphocytes — a type of white blood cell — lower vitamin B6 and higher homocysteine levels than healthy controls, according to a pilot study. The study, “Increased genomic damage and vitamin B status in inflammatory bowel disease patients: A case-control, prospective, pilot study,” was published in the journal Mutation Research/Genetic Toxicology and Environmental Mutagenesis. Vitamin B deficiency and associated hyperhomocysteinemia — elevated blood level of the amino acid homocysteine – are common in patients with IBD. These patients also often experience inflammation in the gut that leads to the formation of reactive oxygen species — free radicals that may damage DNA, lipids (fat) and proteins — potentially causing genomic damage in cells. The CBMN-Cyt assay is able to analyze the extent of genomic damage in lymphocytes by determining the frequency of micronuclei (MNi), small structures with damaged chromosome fragments and/or whole chromosomes that were not incorporated into the nucleus after cell division. MNi are regarded as indicators of genetic damage. Their increase is caused by factors such as vitamin deficiencies, radiation, chronic inflammation, and cancer. The CBMN-Cyt assay has been used to study DNA damage in lymphocytes in diverse disorders; in IBD, such studies are lacking. "To date, only two studies have investigated MNi in patients with IBD: one in pediatric patients and one focusing on patients with UC only," the researchers stated. The team, from South Korea, used this assay to determine the frequency of MNi in lymphocytes and the level of vitamin B in patients with IBD compared with healthy non-smoker controls. A total of 30 adult IBD
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